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Phospho-Tau-S404 polyclonal antibody

货号: BS79354

价格：￥ 1680

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79354

Product Name :Phospho-Tau-S404 polyclonal antibody

Swiss-Prot :P10636

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:100 - 1:200

Background :This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :65kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DDPAC;FTDP-17;MAPTL;MSTD;MTBT1;MTBT2;PPND;PPP1R103;TAU;MAPT;Tau

Immunogen :A synthetic phosphorylated peptide around S404 of human Tau(NP_005901.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of SH-SY5Y and C6 cells, using Phospho-Tau-S404 antibody at 1:2000 dilution. SH-SY5Y cells were treated by Okadaic Acid for 1 hour and Calyculin A for 30 minutes after serum-starvation overnight.C6 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer, using Phospho-Tau-S404 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-Tau-S404 antibody .