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Phospho-ESRα-S118 polyclonal antibody

货号: BS79351

价格：￥ 1680

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79351

Product Name :Phospho-ESRα-S118 polyclonal antibody

Swiss-Prot :P03372

Host :Rabbit

Reactivity :Human

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:100 - 1:200

Background :This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :66kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ESR1;ER;ESR;ESRA;ESTRR;Era;NR3A1;ER alpha

Immunogen :A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1)(NP_000116.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of MCF7 cells, using Phospho-ESRα-S118 pAb at 1:1000 dilution.MCF7 cells were treated by Calyculin A at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-ESRα-S118 antibody .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-ESRα-S118 antibody .