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Phospho-SQSTM1/p62-S349 polyclonal antibody

货号: BS79343

价格：￥ 1680

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS79343

Product Name :Phospho-SQSTM1/p62-S349 polyclonal antibody

Swiss-Prot :Q13501

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated

BiowMW :62KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SQSTM1;A170;DMRV;FTDALS3;NADGP;OSIL;PDB3;ZIP3;p60;p62;p62B

Immunogen :A synthetic phosphorylated peptide around S349 of human SQSTM1/p62(NP_003891.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.NIH/3T3 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.HeLa cells and C6 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.