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Acetyl-Histone H4-K5/K8/K12/K16 polyclonal antibody

货号: BS79334

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79334

Product Name :Acetyl-Histone H4-K5/K8/K12/K16 polyclonal antibody

Swiss-Prot :P62805

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Acetylated

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; HIST2H4; HIST2H4A

Immunogen :A synthetic acetylated peptide around K5/K8/K12/K16 of human HistoneH4.

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.NIH/3T3 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Western blot analysis of extracts of C6 cells, using Acetyl-Histone H4-K5/K8/K12/K16 antibody at 1:520 dilution.C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human liver using Acetyl-Histone H4-K5/K8/K12/K16 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.