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Acetyl-Histone H3-K27 polyclonal antibody

货号: BS79331

价格：￥ 1680

货期：现货（1-2个工作日）

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No. :BS79331

Product Name :Acetyl-Histone H3-K27 polyclonal antibody

Swiss-Prot :P68431

Host :Rabbit

Reactivity :Human, Mouse, Rat, Other (Wide Range)

Applications :DB, WB, ChIP

Application_all :DB,1:500 - 1:2000|WB,1:500 - 1:2000|ChIP,1:50 - 1:200

Background :Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Acetylated

BiowMW :17KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A

Immunogen :A synthetic acetylated peptide around K27 of human Histone H3(NP_003520.1).

Conjugate :Unconjugated

Modification :Acetylated

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody at 1:1000 dilution.NIH/3T3 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody at 1:1000 dilution.HeLa cells and C6 cells were treated by TSA at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K27 antibody at 1:1000 dilution.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.