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ANAPC2 polyclonal antibody

货号: BS78989

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78989

Product Name :ANAPC2 polyclonal antibody

Swiss-Prot :Q9UJX6

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB,IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :102kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ANAPC2;APC2

Immunogen :A synthetic peptide of human ANAPC2(NP_037498.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using ANAPC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat lung using ANAPC2 Rabbit pAb at dilution of 1:350 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat spleen using ANAPC2 Rabbit pAb at dilution of 1:350 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.