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MR1 polyclonal antibody

货号: BS78984

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78984

Product Name :MR1 polyclonal antibody

Swiss-Prot :Q95460

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :39KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MR1;HLALS

Immunogen :Recombinant fusion protein of human MR1(NP_001522.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MR1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of NIH/3T3 cells using MR1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using MR1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.