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MAP2K4 polyclonal antibody

货号: BS78912

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS78912

Product Name :MAP2K4 polyclonal antibody

Swiss-Prot :P45985

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:1000 - 1:4000|IP,1:50 - 1:100

Background :This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :44KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1

Immunogen :A synthetic peptide of human MAP2K4(NP_003001.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of HeLa, using MAP2K4 antibody at 1:750 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody . Western blot was performed from the immunoprecipitate using MAP2K4 antibody at a dilution of 1:1000.