Western blot analysis of extracts of various cell lines, using KDM5A antibody at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody . Western blot was performed from the immunoprecipitate using KDM5A antibody at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.