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KDM5A polyclonal antibody

货号: BS78845
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78845
Product Name :KDM5A polyclonal antibody
Swiss-Prot :P29375
Host :Rabbit
Reactivity :Human, Rat
Applications :WB, IP, ChIP
Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200|ChIP,1:50 - 1:200
Background :This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :192kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :KDM5A;RBBP-2;RBBP2;RBP2
Immunogen :Recombinant fusion protein of human KDM5A.
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using KDM5A antibody at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KDM5A antibody . Western blot was performed from the immunoprecipitate using KDM5A antibody at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com