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[KO Validated] NRBF2 polyclonal antibody

货号: BS78758

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS78758

Product Name :[KO Validated] NRBF2 polyclonal antibody

Swiss-Prot :Q96F24

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IHC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IHC,1:50 - 1:200

Background :May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :38KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NRBF2;COPR;COPR1;COPR2;NRBF-2

Immunogen :Recombinant fusion protein of human NRBF2(NP_110386.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HepG2 cells, using NRBF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3min.

Western blot analysis of extracts from normal and NRBF2 knockout 293T cells, using NRBF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human esophageal using NRBF2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using NRBF2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.