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EIF4G1 polyclonal antibody

货号: BS78723
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78723
Product Name :EIF4G1 polyclonal antibody
Swiss-Prot :Q04637
Host :Rabbit
Reactivity :Human
Applications :WB, IHC
Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200
Background :The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :260kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :EIF4G1;EIF-4G1;EIF4F;EIF4G;EIF4GI;P220;PARK18
Immunogen :A synthetic peptide of human EIF4G1(NP_886553.3).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using EIF4G1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60S.
Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com