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EIF4G1 polyclonal antibody

货号: BS78723

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78723

Product Name :EIF4G1 polyclonal antibody

Swiss-Prot :Q04637

Host :Rabbit

Reactivity :Human

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200

Background :The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :260kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EIF4G1;EIF-4G1;EIF4F;EIF4G;EIF4GI;P220;PARK18

Immunogen :A synthetic peptide of human EIF4G1(NP_886553.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using EIF4G1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60S.

Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.