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CNOT1 polyclonal antibody

货号: BS78714
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78714
Product Name :CNOT1 polyclonal antibody
Swiss-Prot :A5YKK6
Host :Rabbit
Reactivity :Human, Mouse
Applications :WB, IF/ICC
Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200
Background :Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Involved in degradation of AU-rich element (ARE-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES cell identity.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :267kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :CNOT1;AD-005;CDC39;NOT1;NOT1H
Immunogen :Recombinant fusion protein of human CNOT1(NP_057368.3).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell line, using CNOT1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Immunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com