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NCAPH polyclonal antibody

货号: BS78574

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78574

Product Name :NCAPH polyclonal antibody

Swiss-Prot :Q15003

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:5000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :85kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NCAPH;BRRN1;CAP-H

Immunogen :Recombinant fusion protein of human NCAPH(NP_056156.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using NCAPH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.