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TLN1 polyclonal antibody

货号: BS78529

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78529

Product Name :TLN1 polyclonal antibody

Swiss-Prot :Q9Y490

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :240kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TLN1;ILWEQ;TLN;talin-1

Immunogen :Recombinant fusion protein of human TLN1(NP_006280.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of U-87MG cells, using TLN1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 20s.

Immunofluorescence analysis of rat Intestine using TLN1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human colon carcinoma using TLN1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.