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[KO Validated] EIF2AK1 polyclonal antibody

货号: BS78452

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78452

Product Name :[KO Validated] EIF2AK1 polyclonal antibody

Swiss-Prot :Q9BQI3

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :74KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EIF2AK1;HCR;HRI

Immunogen :Recombinant fusion protein of human EIF2AK1(NP_055228.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using EIF2AK1 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Western blot analysis of extracts from wild type and EIF2AK1 Rabbit pAb knockout 293T cells, using EIF2AK1 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.