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HTRA2 polyclonal antibody

货号: BS7842

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7842

Product Name :HTRA2 polyclonal antibody

Swiss-Prot :O43464

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:100

Background :This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :36kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HTRA2;MGCA8;OMI;PARK13;PRSS25

Immunogen :Recombinant fusion protein of human HTRA2(NP_037379.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HTRA2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug HTRA2 antibody . Western blot was performed from the immunoprecipitate using HTRA2 antibody at a dilution of 1:1000.