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Rad50 polyclonal antibody

货号: BS78417
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78417
Product Name :Rad50 polyclonal antibody
Swiss-Prot :Q92878
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IP
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:20 - 1:50
Background :The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :154KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :RAD50;NBSLD;RAD502;hRad50
Immunogen :A synthetic peptide of human Rad50(NP_005723.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using Rad50 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using Rad50 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using Rad50 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug Rad50 antibody . Western blot was performed from the immunoprecipitate using Rad50 antibody at a dilution of 1:500.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com