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SFRP1 polyclonal antibody

货号: BS78401
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78401
Product Name :SFRP1 polyclonal antibody
Swiss-Prot :Q8N474
Host :Rabbit
Reactivity :Human, Mouse
Applications :WB, IHC
Application_all :WB,1:100 - 1:500|IHC,1:50 - 1:100
Background :This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :35kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :SFRP1;FRP;FRP-1;FRP1;FrzA;SARP2
Immunogen :Recombinant fusion protein of human SFRP1(NP_003003.3).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using SFRP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using SFRP1 antibody at dilution of 1:50 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com