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APC polyclonal antibody

货号: BS78389
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78389
Product Name :APC polyclonal antibody
Swiss-Prot :P25054
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Background :This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :150KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :APC;BTPS2;DP2;DP2.5;DP3;GS;PPP1R46
Immunogen :Recombinant fusion protein of human APC(NP_000029.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using APC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human lung using APC antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon using APC antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using APC antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com