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Cytokeratin 7 (KRT7) polyclonal antibody

货号: BS78361

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78361

Product Name :Cytokeratin 7 (KRT7) polyclonal antibody

Swiss-Prot :P08729

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :51kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KRT7;CK7;K2C7;K7;SCL;keratin 7;Keratin

Immunogen :Recombinant fusion protein of human Cytokeratin 7 (Cytokeratin 7 (KRT7))(NP_005547.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using Cytokeratin 7 ) antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of human lung cancer cells using Cytokeratin 7 ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse bronchus cells using Cytokeratin 7 ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat bronchus cells using Cytokeratin 7 ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.