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SMYD4 polyclonal antibody

货号: BS78344

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78344

Product Name :SMYD4 polyclonal antibody

Swiss-Prot :Q8IYR2

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:20 - 1:50

Background :Plays a critical role in cardiac development. Acts as a key epigenetic regulator of gene expression during cardiac development via its dual activities as a methyltransferase and negative regulator of HDAC1 (By similarity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :90kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SMYD4;ZMYND21

Immunogen :Recombinant fusion protein of human SMYD4(NP_443160.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SMYD4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody . Western blot was performed from the immunoprecipitate using SMYD4 antibody at a dilution of 1:500.____Detection: ECL Enhanced Kit ._Exposure time: 20s.