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KDM3B polyclonal antibody

货号: BS78337

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78337

Product Name :KDM3B polyclonal antibody

Swiss-Prot :Q7LBC6

Host :Rabbit

Reactivity :Human

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :225KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KDM3B;5qNCA;C5orf7;JMJD1B;NET22

Immunogen :Recombinant fusion protein of human KDM3B(NP_057688.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using KDM3B Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human esophageal cancer using KDM3B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.