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[KD Validated] RHOT1/MIRO1 polyclonal antibody

货号: BS78314
价格 1540
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78314
Product Name :[KD Validated] RHOT1/MIRO1 polyclonal antibody
Swiss-Prot :Q8IXI2
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Background :Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :75KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :RHOT1; ARHT1; MIRO-1; MIRO1; mitochondrial Rho GTPase 1
Immunogen :Recombinant fusion protein of human RHOT1/MIRO1RHOT1/MIRO1.
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of various lysates, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Western blot analysis of Rat testis, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Western blot analysis of extracts from wild type and RHOT1/MIRO1 knockdown 293T cells, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com