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[KD Validated] RHOT1/MIRO1 polyclonal antibody

货号: BS78314

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78314

Product Name :[KD Validated] RHOT1/MIRO1 polyclonal antibody

Swiss-Prot :Q8IXI2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :75KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RHOT1; ARHT1; MIRO-1; MIRO1; mitochondrial Rho GTPase 1

Immunogen :Recombinant fusion protein of human RHOT1/MIRO1RHOT1/MIRO1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of Rat testis, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Western blot analysis of extracts from wild type and RHOT1/MIRO1 knockdown 293T cells, using RHOT1/MIRO1 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.