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[KO Validated] MTA2 polyclonal antibody

货号: BS78308

价格：￥ 1540

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS78308

Product Name :[KO Validated] MTA2 polyclonal antibody

Swiss-Prot :O94776

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, ChIP

Application_all :WB,1:500 - 1:2000|ChIP,1:50 - 1:200

Background :This gene encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacetylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :75KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MTA2;MTA1L1;PID

Immunogen :Recombinant fusion protein of human MTA2(NP_004730.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and MTA2 knockout 293T cells, using MTA2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.