产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

KAT2A polyclonal antibody

货号: BS78301

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78301

Product Name :KAT2A polyclonal antibody

Swiss-Prot :Q92830

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IHC

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200|IHC,1:50 - 1:200

Background :KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :94KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KAT2A;GCN5;GCN5L2;PCAF-b;hGCN5

Immunogen :Recombinant fusion protein of human KAT2A(NP_066564.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat brain, using KAT2A antibody at 1:462 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse brain using KAT2A Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse heart using KAT2A Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat lung using KAT2A Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.