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[KD Validated] SFRP2 polyclonal antibody

货号: BS78288

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78288

Product Name :[KD Validated] SFRP2 polyclonal antibody

Swiss-Prot :Q96HF1

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :33KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SFRP2;FRP-2;SARP1;SDF-5

Immunogen :A synthetic peptide of human SFRP2(NP_003004.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and SFRP2 knockdown HeLa cells, using SFRP2 antibody at 1:1616 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of A431 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KD Validated] SFRP2 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.