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TIFY10A polyclonal antibody

货号: BS78282

价格：￥ 1955

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78282

Product Name :TIFY10A polyclonal antibody

Swiss-Prot :Q9LMA8

Host :Rabbit

Reactivity :Arabidopsis thaliana

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :JAZ1 is a nuclear-localized protein involved in jasmonate signaling. JAZ1 transcript levels rise in response to a jasmonate stimulus. JAZ1 can interact with the COI1 F-box subunit of an SCF E3 ubiquitin ligase in a yeast-two-hybrid assay only in the presence of jasmonate-isoleucine (JA-ILE) or coronatine. Application of jasmonate methyl ester to Arabidopsis roots reduces the levels of a JAZ1:GUS fusion protein, presumably by stimulating ubiquitin-proteasome-mediated degradation. The Jas domain appears to be important for JAZ1-COI1 interactions in the presence of coronatine. Two positive residues (R205 and R206) in the Jas domain shown to be important for coronatine -dependent COI1 binding are not required for binding AtMYC2.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :32KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AtJAZ1; jasmonate-zim-domain protein 1; T29M8.5; T29M8_5; TIFY10A

Immunogen :A synthetic peptide of arabidopsis thaliana TIFY10A(NP_564075.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rosette leaf, using TIFY10A antibody at 1:1784 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.