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[KO Validated] PEPCK/PCK2 polyclonal antibody

货号: BS78197

价格：￥ 1540

货期：现货（1-2个工作日）

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No. :BS78197

Product Name :[KO Validated] PEPCK/PCK2 polyclonal antibody

Swiss-Prot :Q16822

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB, IF/ICC, IHC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IHC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :71KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PCK2; PEPCK; PEPCK-M; PEPCK2; phosphoenolpyruvate carboxykinase 2, mitochondrial

Immunogen :Recombinant fusion protein of human PEPCK/PCK2(NP_004554.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using PEPCK/PCK2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Western blot analysis of extracts from wild type and PEPCK/PCK2 Rabbit pAb knockout 293T cells, using PEPCK/PCK2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunohistochemistry of paraffin-embedded mouse kidney using PEPCK/PEPCK/PCK2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat kidney using PEPCK/PEPCK/PCK2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.