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[KD Validated] NDUFS5 polyclonal antibody

货号: BS78192

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78192

Product Name :[KD Validated] NDUFS5 polyclonal antibody

Swiss-Prot :O43920

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB,IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :13KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NDUFS5; CI-15k; CI15K; NADH:ubiquinone oxidoreductase subunit S5

Immunogen :Recombinant fusion protein of human NDUFS5(NP_004543.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and NDUFS5 knockdown 293T cells, using NDUFS5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of L929 cells using NDUFS5 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.