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[KO Validated] MSH2 polyclonal antibody

货号: BS78188
价格 1540
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS78188
Product Name :[KO Validated] MSH2 polyclonal antibody
Swiss-Prot :P43246
Host :Rabbit
Reactivity :Human, Rat
Applications :WB, IF/ICC, IP
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200
Background :This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :105KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :MSH2; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; mutS homolog 2
Immunogen :Recombinant fusion protein of human MSH2(NP_000242.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of A-549, using MSH2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Western blot analysis of extracts from wild type and MSH2 Rabbit pAb knockout HeLa cells, using MSH2 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MSH2 antibody . Western blot was performed from the immunoprecipitate using MSH2 antibody at a dilution of 1:3000.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com