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Placental alkaline phosphatase (PLAP) polyclonal antibody

货号: BS78166

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS78166

Product Name :Placental alkaline phosphatase (PLAP) polyclonal antibody

Swiss-Prot :P05187

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,50 - 1:200

Background :The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :Refer to figures

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ALPP; ALP; PALP; PLAP; PLAP-1; alkaline phosphatase, placental

Immunogen :Recombinant fusion protein of human Placental alkaline phosphatase (PLAP)(NP_001623.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using Placental alkaline phosphatase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Placental alkaline phosphatase antibody . Western blot was performed from the immunoprecipitate using Placental alkaline phosphatase antibody at a dilution of 1:1000.