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[KO Validated] NDRG1 polyclonal antibody

货号: BS78144

价格：￥ 1540

货期：现货（1-2个工作日）

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No. :BS78144

Product Name :[KO Validated] NDRG1 polyclonal antibody

Swiss-Prot :Q92597

Host :Rabbit

Reactivity :Human

Applications :IF/ICC

Application_all :IF/ICC,1:50 - 1:200

Background :This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :48KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NDRG1; CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; protein NDRG1

Immunogen :Recombinant fusion protein of human NDRG1(NP_001128714.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and NDRG1 knockout hek293T cells, using NDRG1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using NDRG1 antibody . Blue: DAPI for nuclear staining.