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TIR1 polyclonal antibody

货号: BS78132

价格：￥ 1955

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78132

Product Name :TIR1 polyclonal antibody

Swiss-Prot :Q570C0

Host :Rabbit

Reactivity :Arabidopsis thaliana

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Encodes an auxin receptor that mediates auxin-regulated transcription. It contains leucine-rich repeats and an F-box and interacts with ASK1, ASK2 and AtCUL1 to form SCF-TIR1, an SCF ubiquitin ligase complex. Related to yeast Grr1p and human SKP2 proteins, involved in ubiquitin-mediated processes. Required for normal response to auxin and repressed in response to flagellin. As part of the SCF complex and in the presence of auxin, TIR1 interacts with Aux/IAA transcriptional repressor proteins and mediates their degradation. Mutations in TIR1 block auxin stimulation of flavonoid synthesis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :67KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AtTIR1; TRANSPORT INHIBITOR RESPONSE 1

Immunogen :A synthetic peptide of arabidopsis thaliana TIR1(NP_567135.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Inflorescences, using TIR1 antibody at 1:1660 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.