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SARM1 polyclonal antibody

货号: BS78063

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS78063

Product Name :SARM1 polyclonal antibody

Swiss-Prot :Q6SZW1

Host :Rabbit

Reactivity :Human

Applications :WB,IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :NAD(+ hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+ metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+ depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+ into ADP-D-ribose (ADPR, cyclic ADPR (cADPR and nicotinamide; NAD(+ cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+, but not other NAD(+-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway (By similarity. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :73KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SARM; HsTIR; SAMD2; hSARM1; MyD88-5

Immunogen :A synthetic peptide of human SARM1(NP_055892.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SARM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug SARM1 antibody . Western blot was performed from the immunoprecipitate using SARM1 antibody at a dilution of 1:1000.