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OTULIN polyclonal antibody

货号: BS78035

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS78035

Product Name :OTULIN polyclonal antibody

Swiss-Prot :Q96BN8

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the peptidase C65 family of ubiquitin isopeptidases. Members of this family remove ubiquitin from proteins. The encoded enzyme specifically recognizes and removes M1(Met1)-linked, or linear, ubiquitin chains from protein substrates. Linear ubiquitin chains are known to regulate the NF-kappa B signaling pathway in the context of immunity and inflammation. Mutations in this gene cause a potentially fatal autoinflammatory syndrome in human patients.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :45KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GUM; AIPDS; FAM105B

Immunogen :Recombinant fusion protein of human OTULIN(NP_612357.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat liver, using OTULIN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Western blot analysis of extracts of MCF7 cells, using OTULIN antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using OTULIN Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.