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CCR5 polyclonal antibody

货号: BS77962

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS77962

Product Name :CCR5 polyclonal antibody

Swiss-Prot :P51681

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles; the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :41kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Immunogen :A synthetic peptide of human CCR5(NP_000570.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat liver, using CCR5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of Jurkat cells using CCR5 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using CCR5 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse spleen cells using CCR5 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Fusobacterium nucleatum-driven CX3CR1+ PD-L1+ phagocytes route to tumor tissues and reshape tumor microenvironment

Journal10.1080/19490976.2024.2442037

ApplicationWB

IF12.2

SpeciesHuman

PMID39710592