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C1orf146 polyclonal antibody

货号: BS77864

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS77864

Product Name :C1orf146 polyclonal antibody

Swiss-Prot :Q3KQP7

Host :Rabbit

Reactivity :Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :Plays a key role in reinforcing the integrity of the central element of the synaptonemal complex (SC thereby stabilizing SC, ensuring progression of meiotic prophase I in male and female germ cells. Promotes homologous recombination and crossing-over in meiotic prophase I via its association with SHOC1. Required for the localization of TEX11 and MSH4 to recombination intermediates.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :20KDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Recombinant fusion protein of mouse C1orf146(NP_877422.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat testis, using C1orf146 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of rat testis using C1orf146 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Mouse testis using C1orf146 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse testis using C1orf146 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.