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[KO Validated] Calpain 2 polyclonal antibody

货号: BS77835

价格：￥ 1540

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS77835

Product Name :[KO Validated] Calpain 2 polyclonal antibody

Swiss-Prot :P17655

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :80KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CAPN2;CANP2;CANPL2;CANPml;mCANP;calpain 2

Immunogen :Recombinant fusion protein of human Calpain 2(NP_001739.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and Calpain 2 knockout 293T cells, using Calpain 2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.