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SMC3 polyclonal antibody

货号: BS77708

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS77708

Product Name :SMC3 polyclonal antibody

Swiss-Prot :Q9UQE7

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :142kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BAM;BMH;CDLS3;CSPG6;HCAP;SMC3L1;SMC3

Immunogen :Recombinant fusion protein of human SMC3(NP_005436.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SMC3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunoprecipitation analysis of 600ug extracts of Mouse spleen using 3ug SMC3 antibody . Western blot was performed from the immunoprecipitate using SMC3 antibody at a dilution of 1:1000.