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TLE4 polyclonal antibody

货号: BS77675

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS77675

Product Name :TLE4 polyclonal antibody

Swiss-Prot :Q04727

Host :Rabbit

Reactivity :Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :84kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ESG;BCE1;ESG4;GRG4;BCE-1;Grg-4;E(spI);E(spl);TLE4

Immunogen :Recombinant fusion protein of human TLE4(NP_001269677).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat lung, using TLE4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.