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Tenascin C polyclonal antibody

货号: BS77607

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS77607

Product Name :Tenascin C polyclonal antibody

Swiss-Prot :P24821

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :240KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :150-225;DFNA56;GMEM;GP;HXB;JI;TN;TN-C;TNC

Immunogen :Recombinant fusion protein of human Tenascin C(NP_002151.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of U-138MG, using Tenascin C antibody at 1:1205 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded Human colon carcinoma using Tenascin C antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of U-87MG cells using Tenascin C Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.