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[KO Validated] DIAPH1 polyclonal antibody

货号: BS77584

价格：￥ 1540

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS77584

Product Name :[KO Validated] DIAPH1 polyclonal antibody

Swiss-Prot :O60610

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :150kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DIAPH1;DFNA1;DIA1;DRF1;LFHL1;SCBMS;hDIA1

Immunogen :Recombinant fusion protein of human DIAPH1(NP_001073280.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and DIAPH1 knockout 293T cells, using DIAPH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.