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CD20 polyclonal antibody

货号: BS77560

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS77560

Product Name :CD20 polyclonal antibody

Swiss-Prot :P11836

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :33-37kd

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MS4A1;B1;Bp35;CD20;CVID5;LEU-16;MS4A2;S7

Immunogen :Recombinant fusion protein of human CD20(NP_068769.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Raji cells, using CD20 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human tonsil using CD20 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Raji cells using CD20 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.