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Enpp1 polyclonal antibody

货号: BS77546
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS77546
Product Name :Enpp1 polyclonal antibody
Swiss-Prot :G3X9S2
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Background :This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :120kDa/170kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Pca;ttw;twy;M6S1;NPP1;Npps;PC-1;Ly-41;Pca-1;Pdnp1;C76301;CD203c;E-NPP1;E-NPP 1;AI428932;4833416E15Rik;Enpp1
Immunogen :Recombinant fusion protein of mouse Enpp1(NP_032839.3).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using Enpp1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Immunofluorescence analysis of C6 cells using Enpp1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using Enpp1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com