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Axl polyclonal antibody

货号: BS77544

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS77544

Product Name :Axl polyclonal antibody

Swiss-Prot :P30530

Host :Rabbit

Reactivity :Human

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100

Background :The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :140kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AXL;ARK;JTK11;Tyro7;UFO

Immunogen :Recombinant fusion protein of human AXL(NP_068713.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Axl antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human lung cancer using Axl Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of DU145 cells using Axl Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Axl Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.