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CYP7B1 polyclonal antibody

货号: BS77542

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS77542

Product Name :CYP7B1 polyclonal antibody

Swiss-Prot :O75881

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :55KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CYP7B1;CBAS3;CP7B;SPG5A

Immunogen :A synthetic peptide of human CYP7B1(NP_004811.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of A-431, using CYP7B1 antibody at 1:1980 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of NIH/3T3 cells using CYP7B1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CYP7B1 Rabbit pAb at dilution of 1:25 . Blue: DAPI for nuclear staining.