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CHTF18 polyclonal antibody

货号: BS77455

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS77455

Product Name :CHTF18 polyclonal antibody

Swiss-Prot :Q8WVB6

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a protein which is a component of a replication factor C (RFC) complex, which loads proliferating cell nuclear antigen (PCNA) on to DNA during the S phase of cell cycle. The encoded protein may interact with other proteins, including RFC complex 3, to form a clamp loader complex that plays a role in sister chromatid cohesion during metaphase-anaphase transition. [provided by RefSeq, Jan 2016]

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :130kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :C16orf41;C321D2.2;C321D2.3;C321D2.4;CHL12;Ctf18;RUVBL;CHTF18

Immunogen :Recombinant fusion protein of human CHTF18(NP_071375.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using CHTF18 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of C6 cells using CHTF18 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.