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LIMCH1 polyclonal antibody

货号: BS77381

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS77381

Product Name :LIMCH1 polyclonal antibody

Swiss-Prot :Q9UPQ0

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :140kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LIMCH1A;LMO7B;LIMCH1

Immunogen :Recombinant fusion protein of human LIMCH1(NP_001106189.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of LO2 cells, using LIMCH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.