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MRPS7 polyclonal antibody

货号: BS77120
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS77120
Product Name :MRPS7 polyclonal antibody
Swiss-Prot :Q9Y2R9
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IF/ICC
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Background :Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Unmodification
BiowMW :28kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :MRPS7;MRP-S;MRP-S7;RP-S7;RPMS7;S7mt;bMRP27a
Immunogen :Recombinant fusion protein of human MRPS7(NP_057055.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using MRPS7 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Immunofluorescence analysis of C6 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MRPS7 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com