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[KO Validated] NSDHL polyclonal antibody

货号: BS76990

价格：￥ 1540

货期：现货（1-2个工作日）

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No. :BS76990

Product Name :[KO Validated] NSDHL polyclonal antibody

Swiss-Prot :Q15738

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :40KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NSDHL;H105E3;SDR31E1;XAP104

Immunogen :Recombinant fusion protein of human NSDHL(NP_057006.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and NSDHL knockout 293T cells, using NSDHL antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded Human liver cancer using [KO Validated] NSDHL Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Mouse heart using [KO Validated] NSDHL Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using NSDHL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.